Disposable Passive Electrochemical Microfluidic Device for Diagnosis of Congenital Disorders of Glycosylation

A disposable pump-free electrochemical microfluidic device made by using a multilayer lamination technique is proposed for diagnosis of glycosylation disorders. Thanks to the stacking polyethylene terephthalate (PET) films and double adhesive layers, it possible create geometry that allows filling tailored channels in fixed time. In main channel this passive device, necessary steps perform determination transferrin are performed: labeling protein with Os (VI) complex (electrochemical tag), washing water osmium residues detection adsorptive transfer stripping square wave voltammetry. Electrochemical released two signals: one from due carbohydrates at −0.9 V, other intrinsic signal glycoprotein amino acids +0.8 V. The ratio between them establishes an indicator degree (called index glycosylation). method was successfully applied analysis clinical samples patients congenital disorders glycosylation.